Joined by Horecker's first postdoctoral student, J. Seegmiller, they worked out a new method for the preparation of glucose 6-phosphate and 6-phosphogluconate, both of which were not yet commercially available.As reported in the (JBC) Classic reprinted here, they purified 6-phosphogluconate dehydrogenase from brewer's yeast (1), and by coupling the reduction of TPN to its reoxidation by pyruvate in the presence of lactic dehydrogenase, they were able to show that the first product of 6-phosphogluconate oxidation, in addition to carbon dioxide, was ribulose 5-phosphte.
Periods of elevated temperature have led to the phenomenon of coral bleaching, a disruption of the symbiosis that is characterized by the loss of the algal symbionts or their pigments.
Variations in bleaching responses have been observed that correlate with the identity of the algal symbiont, but the mechanisms behind these differences remain unresolved.
Horecker's personal account of his work on the pentose phosphate pathway can be found in his JBC Reflection (3).
Horecker's contributions to science were recognized with many awards and honors including the Washington Academy of Sciences Award for Scientific Achievement in Biological Sciences (1954) and his election to the National Academy of Sciences in 1961.
This marked the beginning of Horecker's lifelong involvement with the pentose phosphate pathway.